RT Journal Article
SR Electronic
T1 Expression of the Rat <em>CYP2A3</em> Gene in Transgenic Mice
JF Drug Metabolism and Disposition
JO Drug Metab Dispos
FD American Society for Pharmacology and Experimental Therapeutics
SP 548
OP 552
DO 10.1124/dmd.30.5.548
VO 30
IS 5
A1 Su, Ting
A1 Zhang, Qing-Yu
A1 Zhang, Jianhua
A1 Swiatek, Pam
A1 Ding, Xinxin
YR 2002
UL http://dmd.aspetjournals.org/content/30/5/548.abstract
AB Rat CYP2A3 and mouse CYP2A5 are predominantly expressed in the olfactory mucosa. CYP2A3 is also expressed in the lung at a low level, whereas CYP2A5 is expressed in several additional tissues. To better understand the transcriptional regulation of the CYP2Agenes, transgenic mice were generated with a full-lengthCYP2A3 gene fragment containing 3.4 kilobases of the 5′-flanking region. CYP2A3 mRNA was detected in the brain and olfactory bulb in four transgenic mouse lines, in the olfactory mucosa in three lines, and in kidney, liver, lung, and small intestine in two lines. Thus, the expression of the CYP2A3 transgene mimicked the tissue distribution pattern of mouse CYP2A5 rather than that of rat CYP2A3. Furthermore, the levels of CYP2A3 mRNA were very low in all lines examined, suggesting that more distal regulatory regions may be involved in the abundant expression of the CYP2A genes in the olfactory mucosa. The American Society for Pharmacology and Experimental Therapeutics