TY - JOUR T1 - Multidrug Resistance P-Glycoprotein 2 Is Essential for the Biliary Excretion of Indocyanine Green JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 634 LP - 637 VL - 29 IS - 5 AU - Liyue Huang AU - Mary Vore Y1 - 2001/05/01 UR - http://dmd.aspetjournals.org/content/29/5/634.abstract N2 - Multidrug resistance P-glycoprotein 2 (Mdr2) is a phospholipid translocator in the canalicular membrane that is essential for the formation of biliary phospholipid vesicles and mixed lipid/bile salt micelles. Incorporation into biliary vesicles and micelles is thought to contribute to the hepatobiliary excretion of certain hydrophobic organic anions, such as indocyanine green (ICG). The present studies characterized the biliary excretion of two hydrophobic organic anions, ICG and estradiol-17β(β-d-glucuronide) (E217G), in the single-pass isolated perfused liver and the biliary excretion of glutathione (GSH) in vivo in wild-type andMdr2−/− female mice. The biliary excretion of ICG (0.4 μmol) was reduced by 90%, while the biliary excretion of total GSH was decreased by 65% in Mdr2−/−mice relative to wild-type mice. In contrast, the biliary excretion of E217G (0.1 μmol) was increased by 30% inMdr2−/− mice. These data indicate that the absence of Mdr2 differentially influences the biliary excretion of these organic anions and suggest that phospholipid vesicles and mixed micelles in bile are essential for the biliary excretion of ICG. The American Society for Pharmacology and Experimental Therapeutics ER -