TY - JOUR T1 - METABOLISM OF 1-(<em>o</em>-CHLOROPHENYL)-1-(<em>p</em>-CHLOROPHENYL)-2,2-DICHLOROETHANE (<em>o,p</em>’-DDD) IN RATS JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 15 LP - 25 VL - 3 IS - 1 AU - V. D. REIF AU - J. E. SINSHEIMER Y1 - 1975/01/01 UR - http://dmd.aspetjournals.org/content/3/1/15.abstract N2 - The metabolism of 14C-labeled 1-(o-chlorophenyl)-1-(p-chlorophenyl)-2,2-dichloroethane (o,p’-DDD) in rats was investigated. Metabolites were identified in feces and urine extracts by thin-layer chromatography (TLC), gas-liquid chromatography, and mass spectrometry. Extracts of acidic metabolites were methylated with diazomethane for identification. Metabolites were quantitated by TLC and liquid scintillation counting. After a 100-mg oral dose to each of three rats, an average of 7.1% of the radioactivity was excreted in the urine and 87.8% in the feces within 8 days. The urine was found to contain o,p’-dichlorodiphenylacetic acid (o,p’-DDA) as well as 4-hydroxy-, 3-hydroxy-, and 3,4-dihydroxy-substituted o,p’-DDA. The serine and glycine conjugates of o,p’-DDA were also identified. In addition to the above metabolites the feces contained o,p’-DDD, 1-(o-chlorophenyl)-1-(p-chlorophenyl)-2-chloroethylene, and the aspartic acid conjugate of o,p’-DDA. The presence of aromatic mono- and dihydroxylated o,p’-DDD was also detected in feces. Copyright © 1975 by The American Society for Pharmacology and Experimental Therapeutics ER -