PT - JOURNAL ARTICLE AU - Jianguo Liu AU - Spencer S. Ericksen AU - Dan Besspiata AU - Charles W. Fisher AU - Grazyna D. Szklarz TI - Characterization of Substrate Binding to Cytochrome P450 1A1 Using Molecular Modeling and Kinetic Analyses: Case of Residue 382 AID - 10.1124/dmd.31.4.412 DP - 2003 Apr 01 TA - Drug Metabolism and Disposition PG - 412--420 VI - 31 IP - 4 4099 - http://dmd.aspetjournals.org/content/31/4/412.short 4100 - http://dmd.aspetjournals.org/content/31/4/412.full SO - Drug Metab Dispos2003 Apr 01; 31 AB - Key residue Val-382 in P450 1A1 has been predicted to interact with the alkoxy chain of resorufin derivatives. Therefore, we undertook a detailed analysis of substrate mobility in the active site of the P450 1A1 homology model and assessed the effect of mutations at position 382. Dynamic trajectories of 7-methoxy-, 7-ethoxy-, and 7-pentoxyresorufin indicated that 7-ethoxyresorufin would be oxidized most efficiently by the wild-type enzyme. The Val-382→Ala mutation would increase the O-dealkylation of 7-pentoxyresorufin but decrease the oxidation of other substrates. In the case of the V382L mutant, the large bulk of Leu would block alkoxyresorufins from productive binding orientations leading to lowered activities. Binding free energy calculations for three substrates with 1A1 WT and two mutants indicated that binding constants would be similar for all enzyme-substrate combinations. Modeling predictions were tested experimentally. The plasmid containing the cDNA for human P450 1A1 modified for bacterial expression was altered to include a C-terminal PCR-generated six histidine domain to facilitate enzyme purification. The V382A and V382L mutants were constructed by site-directed mutagenesis and Escherichia coli–expressed enzymes purified using Ni-NTA affinity chromatography. The activity of the WT 1A1 was highest toward 7-ethoxyresorufin and lowest toward 7-pentoxyresorufin. Both mutants displayed a decrease inVmax with 7-methoxy- and 7-ethoxyresorufin, whereas for the V382A mutant, Vmax with 7-pentoxyresorufin was increased. No significant changes inKm were observed relative to the wild-type enzyme. The experimental results are thus in good agreement with modeling predictions. The American Society for Pharmacology and Experimental Therapeutics