TY - JOUR T1 - STRUCTURAL AND FUNCTIONAL DIVERSITY IN HEME MONOOXYGENASES JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 10 LP - 18 DO - 10.1124/dmd.104.002071 VL - 33 IS - 1 AU - Thomas L. Poulos Y1 - 2005/01/01 UR - http://dmd.aspetjournals.org/content/33/1/10.abstract N2 - Recent advances in understanding structure-function relationships in cytochrome P450 (P450), nitric-oxide synthase (NOS), and heme oxygenase are summarized. Of particular importance is the role that dynamics plays in P450 function, where the active site undergoes large open/close motions to enable substrates to bind and products to leave. In sharp contrast, the heme-containing active site of NOS is rigid and remains relatively exposed compared with P450s. This difference in dynamics and active site exposure requires that the O2 activation machinery operate somewhat differently in P450 and NOS. Owing to the open NOS active site, the NOS-oxy complex could be subject to nonspecific protonation that short-circuits the normal reaction path. One working hypothesis holds that NOS recruited the cofactor, tetrahydrobiopterin, to bind near the heme for very rapid coupled electron/proton transfer to the oxy complex, which avoids indiscriminate reaction with bulk solvent. Despite these differences, P450, NOS, and also heme oxygenase use a very similar network of H-bonded water molecules in the active site that are required for oxygen activation. Both P450 and NOS are important drug targets. With NOS, the structural basis for isoform-selective inhibition by a class of dipeptide inhibitors has been worked out, thus providing the basis for structure-based drug design. The American Society for Pharmacology and Experimental Therapeutics ER -