PT  - JOURNAL ARTICLE
AU  - Priyamvada Chandra
AU  - Brendan M. Johnson
AU  - Peijin Zhang
AU  - Gary M. Pollack
AU  - Kim L. R. Brouwer
TI  - MODULATION OF HEPATIC CANALICULAR OR BASOLATERAL TRANSPORT PROTEINS ALTERS HEPATOBILIARY DISPOSITION OF A MODEL ORGANIC ANION IN THE ISOLATED PERFUSED RAT LIVER
AID  - 10.1124/dmd.105.003665
DP  - 2005 Aug 01
TA  - Drug Metabolism and Disposition
PG  - 1238--1243
VI  - 33
IP  - 8
4099  - http://dmd.aspetjournals.org/content/33/8/1238.short
4100  - http://dmd.aspetjournals.org/content/33/8/1238.full
SO  - Drug Metab Dispos2005 Aug 01; 33
AB  - This study examined the impact of hepatic transport protein modulation on the hepatobiliary disposition of a nonmetabolized probe substrate, 5- (and 6)-carboxy-2′,7′dichlorofluorescein (CDF) in rat isolated perfused livers (IPLs). In vivo treatment with modulators (100 and 200 mg/kg/day clofibric acid, 80 mg/kg/day phenobarbital, and 25 mg/kg/day dexamethasone) was used to alter the expression of hepatic transport proteins [organic anion transporting polypeptide 1a1, multidrug resistance-associated protein (Mrp) 3, and Mrp2] governing the disposition of CDF. The basolateral and biliary excretion of CDF was measured in single-pass IPLs from control and treated rats. Modulators increased the percentage of CDF eliminated into perfusate of IPLs from treated rats (∼20-35%) compared with controls (∼10%); CDF biliary excretion was decreased in the treated groups. These observations are consistent with modulator-associated increases in the first-order rate constant governing CDF excretion from the hepatocytes into perfusate (kperfusate) or decreases in the first-order rate constant governing CDF excretion into bile (kbile). Pharmacokinetic modeling of the data and subsequent simulations revealed that the routes of CDF excretion were most sensitive to changes in kperfusate. In contrast, hepatic accumulation of CDF was most sensitive to kbile. The differential sensitivity of CDF excretory routes and hepatic accumulation to these rate constants is a function of intrahepatic distribution kinetics, which must be taken into consideration in assessing the potential impact of altered hepatobiliary transport processes. The American Society for Pharmacology and Experimental Therapeutics