PT  - JOURNAL ARTICLE
AU  - H. A. KLAUSNER
AU  - H. G. WILCOX
AU  - J. V. DINGELL
TI  - THE USE OF ZONAL ULTRACENTRIFUGATION IN THE
INVESTIGATION OF THE BINDING OFΔ
<sup>9</sup>-TETRAHYDROCANNABINOL BY PLASMA LIPOPROTEINS
DP  - 1975 Jul 01
TA  - Drug Metabolism and Disposition
PG  - 314--319
VI  - 3
IP  - 4
4099  - http://dmd.aspetjournals.org/content/3/4/314.short
4100  - http://dmd.aspetjournals.org/content/3/4/314.full
SO  - Drug Metab Dispos1975 Jul 01; 3
AB  - The major classes of lipoprotein, very low density, low density, and high density, as well as the lipoprotein-free protein were isolated from plasma by rate-zonal centrifugation. Fractionation of plasma after the addition of Δ9-tetrahydrocannabinol (THC) showed that over 60% of the drug is associated with the lipoprotein; the remainder of the drug appears to be bound by albumin. In human plasma the low density lipoprotein is the major lipoprotein; in rat plasma the very low density and high density lipoproteins predominate. The distribution of THC in the lipoprotein reflected this species difference. In both species the distribution of THC among the lipoproteins appears to be related to their content of neutral lipid or total lipid rather than that of phospholipid or protein. Fractionation of plasma after the addition of estradiol, imipramine, prostaglandin E2, digitoxin, or dicumarol demonstrated that the lipoproteins contribute little to their binding. Thus, even among lipid-soluble compounds, the binding of THC in plasma is unusual. Copyright © 1975 by The American Society for Pharmacology and Experimental Therapeutics