PT  - JOURNAL ARTICLE
AU  - Frank Pietruck
AU  - Markus Horbelt
AU  - Thorsten Feldkamp
AU  - Katrin Engeln
AU  - Stefan Herget-Rosenthal
AU  - Thomas Philipp
AU  - Andreas Kribben
TI  - DIGITAL FLUORESCENCE IMAGING OF ORGANIC CATION TRANSPORT IN FRESHLY ISOLATED RAT PROXIMAL TUBULES
AID  - 10.1124/dmd.105.006403
DP  - 2006 Mar 01
TA  - Drug Metabolism and Disposition
PG  - 339--342
VI  - 34
IP  - 3
4099  - http://dmd.aspetjournals.org/content/34/3/339.short
4100  - http://dmd.aspetjournals.org/content/34/3/339.full
SO  - Drug Metab Dispos2006 Mar 01; 34
AB  - The secretion of cationic drugs and endogenous metabolites is a major function of the kidney. This is accomplished by organic cation transport systems, mainly located in the proximal tubules. Here, we describe a model for continuous measurement of organic cation (OC) transport. In this model, organic cation transport in individual freshly isolated rat proximal tubules is investigated by use of digital fluorescence imaging. To directly measure organic cation transport across the basolateral membrane, the fluorescent organic cation 4-(4-dimethylaminostyryl)-N-methylpyridinium (ASP+) is used with a customized perfusion chamber. ASP+ uptake in this model displayed the characteristics of organic cation transport. Over the tested range of 1 to 50 μM, it showed a concentration-dependent uptake across the basolateral membrane. In the presence of competitive inhibitors of OC transport such as N1-methylnicotinamide+, tetraethylammonium+, and choline+, a concentration-dependent and reversible inhibition of ASP+ uptake could be documented. In conclusion, continuous measurement of organic cation transport in freshly isolated rat proximal tubules by digital fluorescence imaging using ASP+ is a useful tool for investigation of drug transport and interactions and, furthermore, may be helpful for investigation of organic cation transport under pathophysiological conditions. The American Society for Pharmacology and Experimental Therapeutics