TY - JOUR T1 - BENZO[<em>A</em>]PYRENE-INDUCED ORAL CARCINOGENESIS AND CHEMOPREVENTION: STUDIES IN BIOENGINEERED HUMAN TISSUE JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 346 LP - 350 DO - 10.1124/dmd.105.007948 VL - 34 IS - 3 AU - Thomas Walle AU - U. Kristina Walle AU - David Sedmera AU - Mitch Klausner Y1 - 2006/03/01 UR - http://dmd.aspetjournals.org/content/34/3/346.abstract N2 - Oral cancer, originating from smoking-induced lesions of the basal cells in the complex stratified oral epithelium, is difficult to treat. Early detection of premalignant lesions, e.g., leukoplakia, has suggested the possibility of chemopreventive measures, such as topical application of antimutagenic/antiproliferative dietary or pharmaceutical agents. As an extension of a study in human oral epithelial cell monolayers, we determined the carcinogen, i.e., benzo[a]pyrene (BaP), transport, bioactivation, and DNA binding in a bioengineered human gingival epithelial tissue construct and the chemopreventive effects of dietary polyphenols. Short-term experiments showed that both types of compounds can traverse this tissue as well as be effectively taken up by the tissue. The model cigarette smoke carcinogen BaP very slowly, but to a great extent, accumulated in the tissue with maximal uptake at 24 h. Such exposure clearly resulted in DNA binding of BaP by the tissue. This DNA binding was associated with BaP-induced CYP1B1 as well as CYP1A1 expression, as evidenced by mRNA measurements. Cotreatment of the oral tissue with dietary polyphenols, including resveratrol and quercetin, and BaP, resulted in significant inhibition of the BaP-DNA binding. Using fluorescence microscopy as well as simultaneous autoradiography, we also demonstrated that quercetin indeed penetrates the entire stratified tissue layer, but that quercetin was also oxidized within the cells. Thus, this bioengineered oral tissue construct opens up improved ways of understanding and preventing/treating smoking-induced oral cancer. The American Society for Pharmacology and Experimental Therapeutics ER -