TY - JOUR T1 - Prediction of Metabolic Clearance of Bisphenol A (4,4 ′-Dihydroxy-2,2-diphenylpropane) using Cryopreserved Human Hepatocytes JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 1910 LP - 1915 DO - 10.1124/dmd.107.014787 VL - 35 IS - 10 AU - R. K. Kuester AU - I. G. Sipes Y1 - 2007/10/01 UR - http://dmd.aspetjournals.org/content/35/10/1910.abstract N2 - This study investigated the kinetics of glucuronidation of bisphenol A (BPA; 4,4′-dihydroxy-2,2-diphenylpropane) in cryopreserved human hepatocytes (HCs). Incubation conditions were developed using Sprague-Dawley rat HCs. For determination of the kinetic constants of BPA glucuronidation rates with human HCs, viable HCs (0.125 × 106) were incubated with [14C]BPA (1.3-52 μM) for 10 min. The glucuronidation reaction demonstrated Michaelis-Menten kinetics and yielded a mean Km for males and females of 9 ± 3 and 8 ± 2 μM, respectively. The Vmax values of these reactions were 438 ± 129 pmol/min/106 for male HCs and 480 ± 208 pmol/min/106 for female HCs. The scaled intrinsic clearance (CLint) for male human HCs was 149 ± 67 ml/min/kg (range 53-246) and for female HCs was 165 ± 89 ml/min/kg (range 73-336). Overall, there are no apparent gender differences in the glucuronidation of BPA. These CLint values were then extrapolated to estimate total hepatic metabolic clearance (CLmet) using a nonrestrictive well stirred model. The estimated CLmet value for both male and female HCs was 6 ml/min/kg, which represents 30% of hepatic blood flow. Thus, in vivo clearance seems to depend highly on plasma protein binding. These in vitro results correlate well with in vivo studies in humans, which report extensive glucuronidation of BPA. The American Society for Pharmacology and Experimental Therapeutics ER -