TY - JOUR T1 - Plasma Profiling of Intact Isoflavone Metabolites by High-Performance Liquid Chromatography and Mass Spectrometric Identification of Flavone Glycosides Daidzin and Genistin in Human Plasma after Administration of <em>Kinako</em> JF - Drug Metabolism and Disposition JO - Drug Metab Dispos SP - 1485 LP - 1495 DO - 10.1124/dmd.108.021006 VL - 36 IS - 8 AU - Kaori Hosoda AU - Takashi Furuta AU - Akitomo Yokokawa AU - Kenichiro Ogura AU - Akira Hiratsuka AU - Kazuo Ishii Y1 - 2008/08/01 UR - http://dmd.aspetjournals.org/content/36/8/1485.abstract N2 - The roles of isoflavones in the prevention of several hormone-dependent cancers and osteoporosis are of great interest. Despite many pharmacokinetics studies of the isoflavones, the actual types of conjugates circulating in the body and the position(s) of conjugation sites on the flavone skeleton are still uncertain because, in general, conjugated compounds in biological fluids have been evaluated by measuring the free aglycones obtained after selective enzymatic hydrolysis. Using an high-performance (HPLC)-UV-diode-array detector (DAD) method combined with solid-phase extraction, we have obtained HPLC profiles of isoflavone glycosides [daidzin (Din) and genistin (Gin)] and of intact isoflavone metabolites in human plasma: daidzein, genistein, daizein-7-glucuronide, daidzein-4′-glucuronide, genistein-7-glucuronide, genistein-4′-glucuronide, daidzein-7-sulfate, daidzein-4′-sulfate, genistein-7-sulfate, and genistein-4′-sulfate. We investigated the plasma profile of intact isoflavone metabolites in plasma obtained 1 to-7 h after orally administration of 50 g of kinako (baked soybean powder) to two healthy volunteers. The results of DAD analysis indicated that the main isoflavone metabolite peaks were identified on the HPLC chromatogram. Furthermore, the intact glycosides Din and Gin were detected in 1-h plasma samples by their positive electrospray ionization mass spectra, demonstrating that the glycosides Din and Gin can be absorbed from the gut. The American Society for Pharmacology and Experimental Therapeutics ER -