RT Journal Article
SR Electronic
T1 Stable Expression, Activity, and Inducibility of Cytochromes P450 in Differentiated HepaRG Cells
JF Drug Metabolism and Disposition
JO Drug Metab Dispos
FD American Society for Pharmacology and Experimental Therapeutics
SP 516
OP 525
DO 10.1124/dmd.109.030197
VO 38
IS 3
A1 Sébastien Anthérieu
A1 Christophe Chesné
A1 Ruoya Li
A1 Sandrine Camus
A1 Agustin Lahoz
A1 Laura Picazo
A1 Miia Turpeinen
A1 Ari Tolonen
A1 Jouko Uusitalo
A1 Christiane Guguen-Guillouzo
A1 André Guillouzo
YR 2010
UL http://dmd.aspetjournals.org/content/38/3/516.abstract
AB HepaRG cells possess the unique property to differentiate in vitro and to express various functions of mature hepatocytes, including the major cytochromes P450 (P450s). In the present study, we carefully analyzed mRNA expression and activity of the major P450s and their responsiveness to three prototypical inducers, phenobarbital, rifampicin, and omeprazole, in differentiated HepaRG cell cultures over a 4-week period after low and high seeding. Only minor differences were observed in P450 activities when measured by two cocktails of probe substrates, probably related to the choice and/or concentration of substrates. Similar results were obtained from the two cell seeding conditions. Expression and activities of several P450s were dimethyl sulfoxide-dependent. However, basal P450 expression and activities as well as their responsiveness to the prototypical inducers were well maintained over the 4-week period, and a good correlation was observed between transcript levels and corresponding activities. Thus, CYP1A2, CYP2B6, and CYP3A4 were found to accurately respond to their respective prototypical inducers, i.e., omeprazole, phenobarbital, and rifampicin. Likewise, basal expression of several phase II enzymes, transporters, and nuclear receptors, and response to inducers were also well preserved. More genes were found to be induced in HepaRG cells than in primary human hepatocytes, and no marked variation was noticed between the different passages. Taken together, these data support the conclusion that HepaRG cells represent a promising surrogate to primary human hepatocytes for xenobiotic metabolism and toxicity studies. Copyright © 2010 by The American Society for Pharmacology and Experimental Therapeutics