%0 Journal Article %A R M Welch %A C R Hughes %A R L Deangelis %T Effect of 3-methylcholanthrene pretreatment on the bioavailability of phenacetin in the rat. %D 1976 %J Drug Metabolism and Disposition %P 402-406 %V 4 %N 4 %X A thin-layer chromatographic procedure is described for the quantitative determination of phenacetin and acetaminophen in rat plasma. The method was used to determine the effect of 3-methylcholanthrene (3-MC) on the disposition and bioavailability of phenacetin following its oral and iv administration to rats. Pretreatment with 3-MC decreased the plasma half-life of phenacetin, after iv administration, from 28 min to 4.5 min and reduced the systemic bioavailability of phenacetin, after oral administration, from 45% in control rats to 6% in 3-MC-treated rats. By comparing the plasma levels of phenacetin in the portal circulation with those in the peripheral circulation, following the oral administration of phenacetin, it was concluded that the 7-fold reduction in the bioavailability of phenacetin observed in 3-MC treated rats was caused by a marked increase in the metabolism of phenacetin during its first pass through the liver. %U https://dmd.aspetjournals.org/content/dmd/4/4/402.full.pdf