PT  - JOURNAL ARTICLE
AU  - Furner, R L
AU  - Mellett, L B
AU  - Brown, R K
AU  - Duncan, G
TI  - A method for the measurement of L-phenylalanine mustard in the mouse and dog by high-pressure liquid chromatography.
DP  - 1976 Nov 01
TA  - Drug Metabolism and Disposition
PG  - 577--583
VI  - 4
IP  - 6
4099  - http://dmd.aspetjournals.org/content/4/6/577.short
4100  - http://dmd.aspetjournals.org/content/4/6/577.full
SO  - Drug Metab Dispos1976 Nov 01; 4
AB  - The distribution of L-phenylalanine mustard (L-PAM) was studied in dogs and mice by high-pressure liquid chromatography. Separation of L-PAM from its products of hydrolysis was accomplished with a mu-Bondapak C18 column, a solvent system composed of 2-methoxyethanol/0.1% acetic acid, and solvent programming with a step gradient. Complete separation was effected in less than 15 min. The half-life for disappearance of L-PAM from mouse blood was 41 min, whereas that from dog blood was 29 min. The monohydroxy derivative of L-PAM, L-MOH, disappeared from dog serum with a half-life of 32 min. L-MOH was not detectable in mouse tissue other than blood at times greater than 15 min after injection. In the dog at 4 hr after injection, the tissue/serum concentration ratios were greater than 1 for liver, spleen, intestine, skeletal muscle, urinary bladder and gallbladder. The concentration of L-PAM in the bile was approximately 500 times higher than that in serum.