RT Journal Article
SR Electronic
T1 Cytochrome P450 2S1 is Reduced by NADPH-Cytochrome P450 Reductase
JF Drug Metabolism and Disposition
JO Drug Metab Dispos
FD American Society for Pharmacology and Experimental Therapeutics
SP 944
OP 946
DO 10.1124/dmd.111.039321
VO 39
IS 6
A1 Xiao, Yi
A1 Shinkyo, Raku
A1 Guengerich, F. Peter
YR 2011
UL http://dmd.aspetjournals.org/content/39/6/944.abstract
AB Cytochrome P450 (P450) 2S1 is one of the orphan P450s without a clear physiological function. Controversy has arisen as to whether it can interact with NADPH-P450 reductase and accept electrons. The reduction of 1,4-bis{[2-(dimethylamino-N-oxide)ethyl]amino}-5,8-dihydroxyanthracene-9,10-dione (AQ4N) by P450 2S1 was confirmed, and the NADPH consumption rates were measured aerobically and anaerobically in the absence and presence of the drug. The reduction kinetics of P450 2S1 were rapid, as measured by stopped-flow kinetics. These results confirm that P450 2S1 can be reduced by NADPH-P450 reductase and suggest normal mixed-function oxidase roles of P450 2S1 to be revealed.