PT  - JOURNAL ARTICLE
AU  - U Breyer
AU  - G Schmalzing
TI  - Metabolism and disposition of trifluoperazine in the rat. I. A thin-layer chromatographic method for the measurement of trifluoperazine and its metabolites in rat tissues.
DP  - 1977 Mar 01
TA  - Drug Metabolism and Disposition
PG  - 97--103
VI  - 5
IP  - 2
4099  - http://dmd.aspetjournals.org/content/5/2/97.short
4100  - http://dmd.aspetjournals.org/content/5/2/97.full
SO  - Drug Metab Dispos1977 Mar 01; 5
AB  - A method has been developed for quantitative measurement of trifluoperazine and its metabolites, 7-hydroxytrifluoperazine and desmethyltrifluoperazine, in rat organs. Trifluoperazine sulfoxide could also be assayed, but it proved to represent a very minor part only of total biotransformation products in tissues. Alkalinized tissue homogenates were extracted with di-isopropyl ether. Following removal of the bulk of lipids, the compounds to be quantitated were separated by thin-layer chromatography and measured by ultraviolet reflectance photometry on the plates. In recovery experiments, the method proved to possess a high reproducibility. The sensitivity limit for quantitative determination was about 0.1 nmol per extract, and the limit of detectability was 0.025-0.05 nmol. The applicability of the method was shown by analyzing the tissues of rats that had received 12.3 micronmol of trifluoperazine, ip, per kg.