PT  - JOURNAL ARTICLE
AU  - P C Ruenitz
TI  - Rabbit hepatic microsomal O-demethylation of chlorotrianisene.
DP  - 1978 Nov 01
TA  - Drug Metabolism and Disposition
PG  - 631--636
VI  - 6
IP  - 6
4099  - http://dmd.aspetjournals.org/content/6/6/631.short
4100  - http://dmd.aspetjournals.org/content/6/6/631.full
SO  - Drug Metab Dispos1978 Nov 01; 6
AB  - The in vitro hepatic O-demethylation of the nonsteroidal estrogen chlorotrianisene (CTA) has been studied. The rate of O-demethylation was maximal at 0.4 mM NADPH. Although NADH did not catalyze the reaction alone, it had a synergistic effect in the presence of equimolar amounts of NADPH. Carbon monoxide decreased the rate of O-demethylation. Nicotinamide was found to decrease the O-demethylation rate at a concentration of 40 mM, but had no apparent effect at concentrations of 20 mM or lower. Extracts from incubation mixtures contained one major (mono-O-demethylated) and a minor (bis-O-demethylated) metabolite. Extracts of mixtures containing soluble rather than microsomal enzymes or from mixtures in which microsomal protein had been denatured did not contain these metabolites.