PT - JOURNAL ARTICLE AU - C F Gelijkens AU - P Sandra AU - F Belpaire AU - A P De Leenheer TI - Identification and quantitation of 5-fluorouridine in rat urine after administration of 5-fluorouracil or 5-fluoro-2'-deoxyuridine. DP - 1980 Sep 01 TA - Drug Metabolism and Disposition PG - 363--366 VI - 8 IP - 5 4099 - http://dmd.aspetjournals.org/content/8/5/363.short 4100 - http://dmd.aspetjournals.org/content/8/5/363.full SO - Drug Metab Dispos1980 Sep 01; 8 AB - The ribonucleoside 5-fluorouridine was identified in 24-hr urine samples from rats after ip administration of 5-fluorouracil (200 mg/kg) or 5-fluoro-2'-deoxyuridine (380 mg/kg) by means of thin-layer chromatography, high-pressure liquid chromatography, capillary gas-liquid chromatography and gas-liquid chromatography-mass spectrometry techniques, and by comparison with the synthetic compound. By use of a specific extraction procedure, 5-fluorouridine was then quantitatively determined by capillary gas-liquid chromatography with a nitrogen-selective detector. These measurements indicate that not more than 0.2% of the administered dose was excreted as 5-fluorouridine; lower urinary metabolite levels were found after treatment with 5-fluorouracil than after 5-fluoro-2'-deoxyuridine administration. Following an ip dose of either drug, variable amounts of 2'-deoxyuridine and thymidine were detected in the urine samples.