PT  - JOURNAL ARTICLE
AU  - J P Lehman
AU  - L Ferrin
AU  - C Fenselau
AU  - G S Yost
TI  - Simultaneous immobilization of cytochrome P-450 and glucuronyltransferase for synthesis of drug metabolites.
DP  - 1981 Jan 01
TA  - Drug Metabolism and Disposition
PG  - 15--18
VI  - 9
IP  - 1
4099  - http://dmd.aspetjournals.org/content/9/1/15.short
4100  - http://dmd.aspetjournals.org/content/9/1/15.full
SO  - Drug Metab Dispos1981 Jan 01; 9
AB  - Cytochrome P-450, NADPH-cytochrome P-450 reductase, and glucuronyltransferase were immobilized simultaneously on cyanogen bromide-activated Sepharose from phenobarbital-induced rabbit liver microsomes. The activity of the P-450 system was demonstrated by the N-demethylation of ethylmorphine and the O-demethylation of p-nitroanisole. p-Nitrophenol produced from the oxidation of p-nitroanisole was conjugated to p-nitrophenyl glucuronide, as evidenced by isolation and characterization of the glucuronide by GC/MS.