PT  - JOURNAL ARTICLE
AU  - Rock, Brooke M.
AU  - Tometsko, Mark E.
AU  - Patel, Sonal K.
AU  - Hamblett, Kevin J.
AU  - Fanslow, William C.
AU  - Rock, Dan A.
TI  - Intracellular Catabolism of an Antibody Drug Conjugate with a Noncleavable Linker
AID  - 10.1124/dmd.115.064253
DP  - 2015 Sep 01
TA  - Drug Metabolism and Disposition
PG  - 1341--1344
VI  - 43
IP  - 9
4099  - http://dmd.aspetjournals.org/content/43/9/1341.short
4100  - http://dmd.aspetjournals.org/content/43/9/1341.full
SO  - Drug Metab Dispos2015 Sep 01; 43
AB  - Antibody drug conjugates are emerging as a powerful class of antitumor agents with efficacy across a range of cancers; therefore, understanding the disposition of this class of therapeutic is crucial. Reported here is a method of enriching a specific organelle (lysosome) to understand the catabolism of an anti-CD70 Ab-MCC-DM1, an antibody drug conjugate with a noncleavable linker. With such techniques a higher degree of concentration-activity relationship can be established for in vitro cell lines; this can aid in understanding the resultant catabolite concentrations necessary to exert activity.