TY - JOUR T1 - Inhibition of SULT4A1 Expression Induces Up-regulation of Phototransduction Gene Expression in 72 Hours Post Fertilization Zebrafish Larvae JF - Drug Metabolism and Disposition JO - Drug Metab Dispos DO - 10.1124/dmd.114.057042 SP - dmd.114.057042 AU - Frank Crittenden AU - Holly Thomas AU - Cheryl M Ethen AU - Zhengliang L Wu AU - Dongquan Chen AU - Timothy Kraft AU - John Parant AU - Charles N Falany Y1 - 2014/02/19 UR - http://dmd.aspetjournals.org/content/early/2014/02/19/dmd.114.057042.abstract N2 - Sulfotransferase (SULT) 4A1 is an orphan enzyme that shares distinct structure and sequence similarities with other cytosolic SULTs. SULT4A1 is primarily expressed in neuronal tissue and is also the most conserved SULT, having been identified in every vertebrate investigated to date. Certain haplotypes of the SULT4A1 gene are correlated with higher baseline psychopathology in schizophrenic patients, but no substrate or function for SULT4A1 has yet been identified despite its high level of sequence conservation. In this study, RNA-seq was used to search for alterations in gene expression in 72 hours post fertilization (hpf) zebrafish larvae following transient SULT4A1 knockdown (KD) utilizing splice blocking morpholino oligonucleotides (MOs). This study demonstrates that transient inhibition of SULT4A1 expression in developing zebrafish larvae results in the up-regulation of several genes involved in phototransduction. SULT4A1 KD was verified by immunoblot analysis and quantitative real-time PCR (qPCR). Gene regulation changes identified by deep RNA sequencing were validated by qPCR. This study is the first identification of a cellular process whose regulation appears to be associated with SULT4A1 expression. ER -