TY - JOUR T1 - Differential Expression of Cytochrome P450 Enzymes from the CYP2C Subfamily in Human Brain JF - Drug Metabolism and Disposition JO - Drug Metab Dispos DO - 10.1124/dmd.114.061242 SP - dmd.114.061242 AU - Iris M Booth Depaz AU - Francesca Toselli AU - Peter A Wilce AU - Elizabeth MJ Gillam Y1 - 2014/12/11 UR - http://dmd.aspetjournals.org/content/early/2014/12/11/dmd.114.061242.abstract N2 - Cytochrome P450 enzymes from the CYP2C subfamily play a prominent role in the metabolic clearance of many drugs. CYP2C enzymes have also been implicated in the metabolism of arachidonic acid to vasoactive epoxyeicosatrienoic acids. CYP2C8, CYP2C9, and CYP2C19 are expressed in the adult liver at significant levels; however the expression of CYP2C enzymes in extrahepatic tissues such as the brain is less well characterized. Form-specific antibodies to CYP2C9 and CYP2C19 were prepared by affinity purification of antibodies raised to unique peptides. CYP2C9 and CYP2C19 were located in microsomal fractions of all five human brain regions examined, namely frontal cortex, hippocampus, basal ganglia, amygdala and cerebellum. Both CYP2C9 and CYP2C19 were detected predominantly within neuronal soma but with expression extending down axons and dendrites in certain regions. Finally, a comparison of cortex samples from alcoholics and age-matched controls suggested CYP2C9 expression was increased in alcoholics. ER -