TY - JOUR T1 - Hepatic clearance predictions from in vitro-in vivo extrapolation and BDDCS JF - Drug Metabolism and Disposition JO - Drug Metab Dispos DO - 10.1124/dmd.116.071514 SP - dmd.116.071514 AU - Christine M. Bowman AU - Leslie Z. Benet Y1 - 2016/01/01 UR - http://dmd.aspetjournals.org/content/early/2016/08/12/dmd.116.071514.abstract N2 - Predicting in vivo pharmacokinetic parameters such as clearance from in vitro data is a crucial part of the drug development process. There is a commonly cited trend that drugs that are highly protein bound and are substrates for hepatic uptake transporters often yield the worst predictions. Given this information, 11 different data sets using human microsomes and hepatocytes were evaluated to search for trends in accuracy, extent of protein binding, and drug classification based on the Biopharmaceutics Drug Disposition Classification System (BDDCS), which makes predictions about transporter effects. As previously reported, both in vitro systems (microsomes and hepatocytes) gave a large number of inaccurate results, defined as predictions falling more than 2-fold outside of in vivo values. The weighted average of the percentage of inaccuracy was 66.5%. BDDCS class 2 drugs, which are subject to transporter effects in vivo unlike class 1 compounds, had a higher percentage of inaccurate predictions and often had slightly larger bias. However, since the weighted average of the percent inaccuracy was still high in both classes (81.9% for class 2, and 62.3% for class 1), it may be currently hard to use BDDCS class to predict potential accuracy. The results of this study emphasize the need for improved IVIVE experimental methods as using physiologically based scaling is still not accurate, and BDDCS cannot currently help predict accurate results. ER -