RT Journal Article
SR Electronic
T1 Sulfotransferase 4A1 (SULT4A1) increases its expression in mouse neurons as they mature
JF Drug Metabolism and Disposition
JO Drug Metab Dispos
FD American Society for Pharmacology and Experimental Therapeutics
SP dmd.118.080838
DO 10.1124/dmd.118.080838
A1 Takuyu Hashiguchi
A1 Sawako Shindo
A1 Shih-Heng Chen
A1 Jau-Shyong Hong
A1 Masahiko Negishi
YR 2018
UL http://dmd.aspetjournals.org/content/early/2018/04/06/dmd.118.080838.abstract
AB Cytosolic sulfotransferases (SULTs) catalyze sulfation and play essential roles in detoxification of xenobiotics as well as inactivation of endo-biotics. Sulfotransferase 4A1 (SULT4A1) which was originally isolated as a brain-specific sulfotransferase, is the most highly conserved isoform among SULTs in vertebrates. Here, expression of SULT4A1 was examined neuron enriched and neuron-glia mixed cells derived from mouse embryo brains at day 14 gestation and mixed glia from 2 days-old neonate brains. Western blots showed an increase of SULT4A1 expression as neurons maturated. RT-PCR and agarose gel analysis found two different forms (variant and wild type) of SULT4A1 mRNA in neurons; the level of wild type correlates with the protein level of SULT4A1. SULT1E1 was expressed neither in mouse brains, neuron enriched cells nor mixed glia cells. SULT1A1 protein was only detected in adult brains. Immunofluorescence staining of neuron-glia mixed cells confirmed selective expression of SULT4A1 in neurons including dopaminergic neurons, but not in either astrocytes or microglia. Thus, SULT4A1 is a neuron-specific sulfotransferase and may play a role in neuronal development.