@article {Lidmd.118.081547, author = {Cindy Yanfei Li and Joseph L. Dempsey and Dongfang Wang and SooWan Lee and Kris M. Weigel and Qiang Fei and Deepak Kumar Bhatt and Bhagwat Prasad and Daniel Raftery and Haiwei Gu and Julia Yue Cui}, title = {PBDEs Altered Gut Microbiome and Bile Acid Homeostasis in Male C57BL/6 Mice}, elocation-id = {dmd.118.081547}, year = {2018}, doi = {10.1124/dmd.118.081547}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, abstract = {Polybrominated diphenyl ethers (PBDEs) are persistent environmental contaminants with well-characterized toxicities in host organs. Gut microbiome is increasingly recognized as an important regulator of xenobiotic biotransformation; however, little is known about its interactions with PBDEs. Primary bile acids (BAs) are metabolized by the gut microbiome into more lipophilic secondary BAs that may be absorbed and interact with certain host receptors. The goal of this study was to test our hypothesis that PBDEs cause dysbiosis and aberrant regulation of BA homeostasis. Nine-week-old male C57BL/6 conventional (CV) and germ-free (GF) mice were orally gavaged with corn oil (10 mg/kg), BDE-47 (100 μmol/kg), or BDE-99 (100 μmol/kg) once daily for 4-days (n=3-5/group). Gut microbiome was characterized using 16S rRNA sequencing of the large intestinal content in CV mice. Both BDE-47 and BDE-99 profoundly decreased the alpha diversity of gut microbiome and differentially regulated 45 bacterial species. Both PBDE congeners increased Akkermansia muciniphila and Erysipelotrichacea Allobaculum spp., which have been reported to have anti-inflammatory and anti-obesity functions. Targeted metabolomics of 56 BAs was conducted in serum, liver, and small and large intestinal content of CV and GF mice. BDE-99 increased many unconjugated BAs in multiple bio-compartments in a gut microbiota-dependent manner. This correlated with an increase in microbial 7α-dehydroxylation enzymes for secondary BA synthesis and increased expression of host intestinal transporters for BA absorption. Targeted proteomics showed that PBDEs down-regulated host BA-synthesizing enzymes and transporters in livers of CV but not GF mice. In conclusion, there is a novel interaction between PBDEs and the endogenous BA-signaling through modifying the "gut-liver axis".}, issn = {0090-9556}, URL = {https://dmd.aspetjournals.org/content/early/2018/05/16/dmd.118.081547}, eprint = {https://dmd.aspetjournals.org/content/early/2018/05/16/dmd.118.081547.full.pdf}, journal = {Drug Metabolism and Disposition} }