Hydrolysis of temocapril in rat jejunal homogenate and S9 fraction prepared after perfusion with and without BNPP
After perfusion, with or without BNPP (400 μM) for 40 min, homogenates and S9 fractions were prepared from the jejunal mucosa and kept on ice. Intact jejunum was used as the control. The hydrolysis of 500 μM temocapril was measured in samples of homogenate and S9 fraction containing 400 μg/ml protein. Results are expressed as the quantity of temocapril hydrolysed (nmol · min−1 · mg protein−1) and are given as mean ± S.D. (n = 4). The percentage in parentheses indicates the remaining activity to each control.
| Intact Mucosa | Perfused Mucosa | |||
|---|---|---|---|---|
| Homogenate | S9 | Homogenate | S9 | |
| nmol · min−1 · mg protein−1 | ||||
| Control | 4.17 ± 0.428 | 4.46 ± 0.137 | ||
| +100 μM BNPP | 2.23 ± 0.131 (53.4%) | 1.13 ± 0.443 (25.2%) | ||
| +1000 μM BNPP | 1.97 ± 0.194 (47.4%) | 0.488 ± 0.105 (10.9%) | ||
| Control | 3.96 ± 0.056 | 4.28 ± 0.029 | ||
| Perfusion with 400 μM BNPP | 2.05 ± 0.255 (51.8%) | 0.625 ± 0.111 (15.2%) | ||