Description of microsomal incubation conditions
| P450 Isozyme | Substrate/Solvent | Concentration (μM) | Amount of protein (mg) | Incubation Time (min) | Quenching Methodology | Ref. | Inhibitor/Solvent |
|---|---|---|---|---|---|---|---|
| 1A1/1A2 | Phenacetin/methanol | 100 | 0.5 | 20 | 150 μl acetonitrile | 26 | Furafylline/methanol |
| 2A6 | Coumarin/methanol | 50 | 0.75 | 15 | 500 μl acetonitrile | 27 | Monoclonal antibody1-a |
| 2C8/2C9 | Tolbutamide/methanol | 500 | 1 | 120 | 50 μl 85% phosphoric acid | 28 | Sulfaphenazole/methanol |
| 2C19 | S-Mephenytoin/acetonitrile | 200 | 0.3 | 60 | 80 μl 70% perchloric acid | 29,30 | Tranylcypromine/water |
| 2D6 | Dextromethorphan/water | 50 | 1 | 30 or 5 | 80 μl 70% perchloric acid | 31 | Quinidine/water |
| 2E1 | Chlorzoxazone/methanol | 100 | 1 | 30 | 500 μl acetonitrile | 6 | Diethyldithiocarbamate/water |
| 3A4 | Testosterone/methanol | 90 | 1 | 7 | 500 μl acetonitrile | 32 | Troleandomycin/methanol |
↵1-a The antibody and microsomal protein were kept on ice for 15 min before the pre-incubation step.