Kinetics of indinavir metabolism in human liver microsomes obtained from fetal, pediatric and adult subjects
| Human Subjects1-a | Km(μM) | Vmax (pmol/min/mg) | Vmax/Km (μl/min/mg) |
|---|---|---|---|
| Fetus | |||
| HFM-1 | 2.87 | 18.2 | 6.35 |
| HFM-2 | 1.70 | 8.57 | 5.03 |
| HFM-3 | 2.06 | 12.0 | 5.82 |
| Average | 2.21 | 12.9** | 5.73** |
| SD | 0.60 | 4.9 | 0.66 |
| Pediatric | |||
| HPM-1 | 2.57 | 32.0 | 12.5 |
| HPM-2 | 1.33 | 15.7 | 11.8 |
| HPM-3 | 1.10 | 13.6 | 12.4 |
| Average | 1.67 | 20.4* | 12.2** |
| SD | 0.79 | 10.1 | 0.4 |
| Adult | |||
| HAM-1 | 2.18 | 38.8 | 17.7 |
| HAM-2 | 2.76 | 51.2 | 18.5 |
| HAM-3 | 1.98 | 35.4 | 17.9 |
| Average | 2.31 | 41.8 | 18.0 |
| SD | 0.41 | 8.3 | 0.4 |
↵1-a Fetal liver microsomes were obtained at 20 weeks gestation (N = 3). Ages of pediatric and adult subjects were 9.0 ± 2.6 (mean ± SD, N = 3) and 43.7 ± 7.1 (mean ± SD, N = 3), respectively. Significant differences from the corresponding adult subject values are shown by asterisks (*p < 0.05; **p < 0.01).