Table 2

Mass spectrometric and radiometric determination of lauric acid hydroxylation

HPLC Peak No. Fig. 1 Identity% of Total Radioactivity2-a or of Total Ion Current2-a
TroutRat
RadioactivityGC/MSRadioactivityGC/MS
412-OH (ω)1.0  ± 0.32-b 1.2  ± 0.214.0  ± 1.32-b 20.3  ± 2.7
111-OH (ω-1)16.4  ± 3.926.0  ± 4.610.6  ± 1.717.6  ± 2.2
210-OH (ω-2)4.4  ± 1.55.6  ± 1.40.5  ± 0.40.7  ± 0.3
39-OH (ω-3)1.3  ± 0.82.7  ± 1.6ND2-c tr2-c
48-OH (ω-4) 2-b 0.4  ± 0.3 2-b ND
57-OH (ω-5)0.1  ± 0.20.3  ± 0.2ND ND
66-OH (ω-6)2.7  ± 1.94.3  ± 2.6trtr
7lauric acid73.6  ± 3.659.9  ± 4.374.6  ± 2.661.5  ± 3.0

  • ↵2-a Mean ± S.D. for four male animals.

  • ↵2-b The radioactive data includes the contribution of both 8-OH and 12-OH lauric acid as these two metabolites are not resolved in the HPLC separation.

  • ↵2-c ND, not detected; tr, trace amount detected but too low to quantitate.