Correlation of the in vitro N-dehydroxylation of guanoxabenz to guanabenz with known isoenzyme-specific P450 marker activities in 10 different samples of human hepatic microsomes
| Marker Reaction | Responsible P450 Isoenzyme | r |
|---|---|---|
| 7-EthoxyresorufinO-deethylation | CYP1A2 | 0.33 |
| Caffeine N-demethylation | CYP1A2 | 0.36 |
| Coumarin 7-hydroxylation | CYP2A6 | 0.36 |
| Tolbutamide-methylhydroxylation | CYP2C9/10 | 0.48 |
| (S)-Mephenytoin 4′-hydroxylation | CYP2C19 | 0.06 |
| Dextrometorphan O-demethylation | CYP2D6 | 0.14 |
| Chlorzoxazone 6-hydroxylation | CYP2E1 | 0.03 |
| Testosterone 6β-hydroxylation | CYP3A4 | 0.43 |
| Lauric acid 12-hydroxylation | CYP4A | 0.04 |
For incubation procedure, sample work-up, and analysis seeMaterials and Methods.