Glucuronidation of opioids by human UGT2B7(Y) expressed in HK 293 cells
| Substrates | Rate of Glucuronidation |
|---|---|
| pmol/min/mg protein | |
| Morphine | 160 ± 331-a |
| Naloxone | 131 ± 32 |
| Nalorphine | 134 ± 18 |
| Naltrexone | 11.0 ± 4.4 |
| Buprenorphine | 10.2 ± 0.9 |
| Codeine | 1.8 ± 0.4 |
Rates were measured in homogenates of cells from 3 or 4 passages. Assays were conducted as described in Materials and Methodsusing the extraction procedure of Puig and Tephly (9). Incubation time was 60 min using 2 mM UDP-GlcUA and 100 μg protein.
↵1-a Values represent the means ± SD.