Kinetic parameter values for the N-demethylation/N-oxide formation of clozapine by CYP enzymes
| Reaction | Kinetic Parameters | CYP Enzymes | ||||
|---|---|---|---|---|---|---|
| 1A2 | 3A4 | 2C9 | 2C19 | 2D6 | ||
| N-Demethylation | K M(μM) | 25 ± 6.5 | 230 ± 30 | 15 ± 1.3 | 13 ± 2.2 | 25 ± 0.7 |
| V max (mol/hr/mol CYP) | 44 ± 1.5 | 89 ± 6.2 | 27 ± 2.1 | 150 ± 17 | 370 ± 18 | |
| K M/V max(106 liters/hr/mol CYP) | 1.7 ± 0.6 | 0.4 ± 0.02 | 1.7 ± 0.1 | 12 ± 3.1 | 15 ± 0.9 | |
| N-Oxide reaction | K M(μM) | 120 ± 6.9 | ||||
| V max(mol/hr/mol CYP) | 120 ± 0.5 | |||||
| K M/V max(106 liters/hr/mol CYP) | 1.0 ± 0.05 | |||||
Values are mean ± SE (N = 3 independent series).