18O incorporation into hydroquinone formed by the microsomal cytochrome P450 system
| Substituent (X) | 18O Incorporation |
|---|---|
| F | >99 |
| Cl | 82 |
| Br | 90 |
| NO2 | >99 |
| CN | >99 |
| CH2OH | 91 |
| COCH3 | 94 |
| COPh | >99 |
| COOH | >99 |
Hydroquinone was detected as the trimethylsilylated hydroquinone form. The 18O content calculated from the 256/254 peak ratio in the mass spectrum of the trimethylsilylated hydroquinone and then corrected on the basis of the original 18O content of18O2.