Table 1

Kinetic parameters for the 7-hydroxylation of coumarin in a panel of 12 human liver microsomal preparations1-a

LiverAgeSexEthnicity1-bDrug History1-cPathologyKmdVmax1-e
10946MCaa, IFatty liver0.625.28
11924MCDNone0.493.35
12738MCNoneNone0.441.09
12936MCC, Pred, NNone0.318.01
13162FCPraz, L, D, PNone0.3913.99
1347MCNoneNone0.434.69
13545MCUFatty liver0.304.33
13711MAAPNone0.314.04
1389FCNoneAcute liver injury0.160.64
13915FCPNone0.5522.58
14610MCNoneNone0.517.11
14963FCaaFibrotic0.340.97
Mean ± SD0.40 ± 0.136.34
Variation4-fold36-fold
  • 1-a Values given are the average of duplicate determinations.

  • 1-b AA, African-American; C, Caucasian.

  • 1-c aa, alcohol abuse; I, inderal; D, dexamethasone; C, cyclosporine; Pred, prednisone; N, nifedipine; Praz, prazepam; P, phenytoin; L, lozol; U, urokinase.

  • 1-d Expressed as μM.

  • 1-e Expressed as nmol/nmol P450/min.