Table 2

Inhibitory effects of anti-P450 IgG on hydroxylation of (+)-S-145, bisnor, DH-bisnor, and tetranor by liver microsomes from untreated rats

5-Hydroxylation% of Control2-a
(+)-S-145BisnorDH-bisnorTetranor
Control100100100100
(134)(97)(360)(35)
Preimmune11210311897
Anti-P4503A1/257 ND2-b 4495
Anti-P4502C61087295101
Anti-P4502C111041046186
6-Hydroxylation% of Control2-a
(+)-S-145BisnorDH-bisnorTetranor
Control100100100100
(142)(46)(364)(80)
Preimmune11210611089
Anti-P4503A1/21075797ND
Anti-P4502C644645777
Anti-P4502C1195697884

  • ↵2-a All results are expressed as percentage of control experiments (minus IgG). Each value is the mean of two animals, and the values in parentheses are hydroxylation activities (pmol/min/mg protein) for control assays.

  • ↵2-b ND, not detected (<1 pmol/min/mg protein).