Inhibitory effects of antibodies against recombinant human P450s on hydroxylation of (+)-S-145, bisnor, and tetranor by human liver microsomes
| 5-Hydroxylation | % of Control4-a | ||
|---|---|---|---|
| (+)-S-145 | Bisnor | Tetranor | |
| Control | 100 | 100 | 100 |
| (81, 111) | (83, 148) | (101, 288) | |
| Preimmune | 93 | 99 | 130 |
| Anti-P4501A2 | 99 | 92 | 109 |
| Anti-P4502C9/10 | 68 | 81 | 21 |
| Anti-P4502E1 | 114 | 111 | 157 |
| Anti-P4503A4 | 45 | 47 | 73 |
| 6-Hydroxylation | % of Control4-a | ||
|---|---|---|---|
| (+)-S-145 | Bisnor | Tetranor | |
| Control | 100 | 100 | 100 |
| (96, 334) | (50, 248) | (51, 136) | |
| Preimmune | 103 | 91 | 91 |
| Anti-P4501A2 | 116 | 93 | 66 |
| Anti-P4502C9/10 | 67 | 81 | 67 |
| Anti-P4502E1 | 117 | 109 | 125 |
| Anti-P4503A4 | 96 | 83 | 24 |
Values in parentheses are hydroxylation activities (pmol/min/mg protein) of two microsomal preparations in control assays (no. on left, from HM-2; no. on right, from HM-6).
↵4-a All data are expressed as the relative percentage of control experiments (minus IgG).