Effects of thawing of human samples and storage at 25°C for 6 hr on the contents of microsomal proteins and P450
| Group | Number of Samples (n) | Liver Microsomal Contents | |
|---|---|---|---|
| Protein (mg/g liver) | P450 (nmol/mg protein) | ||
| Freezing | 16 | 15.1 ± 3.2 | 0.304 ± 0.185 |
| Freezing/thawing/holding at 25°C | 16 | 17.0 ± 5.4 | 0.025 ± 0.039* |
Some (freezing/thawing/holding at 25°C) of the liver samples which were kept at −80°C for 1 month were thawed and kept at 25°C for 6 hr and then refrozen and kept at −80°C for 1 week before preparing microsomes. Other samples (freezing) were kept at −80°C without thawing. Liver microsomes of both groups were prepared at the same time.
* p < 0.01.