Table 1

Hydrolysis of biosynthetic atRAG by β-glucuronidase

SampleatRAG Recovered
pmol
Control172  ± 7 (100)
+β-Glucuronidase45  ± 1 (26)
+β-Glucuronidase + saccharolactone158  ± 6 (92)

Sprague-Dawley microsomes (50 μg of protein) were incubated with 0.1 mM [3H]atRA and 4 mM UDP-GlcUA for 30 min at 37°C. After incubation, reactions were either stopped (control) or incubated for an additional 2 hr with 100 units of β-glucuronidase, in the absence or presence of 12.5 mM saccharolactone. The results are expressed as the mean ± SD of at least two separate duplicate determinations. The numbers in parentheses give the percentage of the control recovery.