Sample | atRAG Recovered |
---|---|
pmol | |
Control | 172 ± 7 (100) |
+β-Glucuronidase | 45 ± 1 (26) |
+β-Glucuronidase + saccharolactone | 158 ± 6 (92) |
Sprague-Dawley microsomes (50 μg of protein) were incubated with 0.1 mM [3H]atRA and 4 mM UDP-GlcUA for 30 min at 37°C. After incubation, reactions were either stopped (control) or incubated for an additional 2 hr with 100 units of β-glucuronidase, in the absence or presence of 12.5 mM saccharolactone. The results are expressed as the mean ± SD of at least two separate duplicate determinations. The numbers in parentheses give the percentage of the control recovery.