Inhibition of 4-ABP co-oxidation catalyzed by SLO and HTPLO
| Inhibitor | Final Conc. (μM) | Rate of 4-ABP Co-oxidation | |
|---|---|---|---|
| SLO, Relative Activity | HTPLO, Relative Activity | ||
| 100 | 100 | ||
| NDGA | 1 | 36 ± 3 | 73 ± 4 |
| 5 | 19 ± 2 | 36 ± 8 | |
| Gossypol | 1 | 45 ± 6 | 69 ± 7 |
| 5 | 29 ± 3 | 32 ± 4 | |
| ETI | 1 | 64 ± 8 | 74 ± 4 |
| 10 | 49 ± 5 | 53 ± 6 | |
| BHT | 1 | 61 ± 4 | |
| 5 | 39 ± 1 | 74 ± 4 | |
| 10 | 51 ± 5 | ||
| BHA | 1 | 64 ± 6 | |
| 5 | 46 ± 4 | 70 ± 3 | |
| 10 | 43 ± 3 | ||
Values indicated are mean ± SEM (N = 4). Control specific activities (shown as 100 relative activity) towards 4-ABP for SLO and HTPLO were 350 ± 41 nmoles of 4-ABP depleted/min/nmole of SLO and 45 ± 6 nmoles of 4-ABP depleted/min/mg protein, respectively.
Reaction mixture (final volume 1 ml) contained 75 pmoles of SLO or 80 μg HTPLO, 50 μM 4-ABP, 2 mM linoleic acid, and indicated concentration of inhibitors in 50 mM Tris buffer, pH 8.5 and pH 7.4, respectively.
All values observed in the presence of inhibitors tested are significantly less than the respective control (p < 0.05), based on ANOVA followed by Fisher’s LSD test.