Apparent kinetic parameters for the N-glucuronidation of amitriptyline and diphenhydramine in microsomes from four human liver samples (mean ± SD)
| Parameter | Substrate | |
|---|---|---|
| Amitriptyline | Diphenhydramine | |
| K M1(μM) | 1.4 ± 0.2 | 2.6 ± 0.9 |
| V max1 (pmol·mg protein−1·min−1) | ||
| HL 15 | 8.1 ± 0.85 | 4.1 ± 1.0 |
| HL 16 | 2.8 ± 0.3 | 1.9 ± 0.3 |
| HL 17 | 15.8 ± 1.0 | 12.3 ± 1.6 |
| HL 19 | 6.9 ± 0.4 | 17.4 ± 1.6 |
| K M2(μM) | 311 ± 23 | 1180 ± 280 |
| V max2 (pmol·mg protein−1·min−1) | ||
| HL 15 | 522 ± 26 | 740 ± 140 |
| HL 16 | 165 ± 9 | 164 ± 31 |
| HL 17 | 250 ± 14 | 388 ± 68 |
| HL 19 | 83 ± 5 | 253 ± 44 |
| V max1/K M1 (μl·mg protein−1·min−1) | ||
| HL 15 | 5.9 | 1.6 |
| HL 16 | 2.1 | 0.7 |
| HL 17 | 11.6 | 4.7 |
| HL 19 | 5.1 | 6.6 |
| V max2/K M2 (μl·mg protein−1·min−1) | ||
| HL 15 | 1.7 | 0.63 |
| HL 16 | 0.53 | 0.14 |
| HL 17 | 0.80 | 0.33 |
| HL 19 | 0.27 | 0.22 |