Table 1

Initial rate constants of hydrolysis and velocities catalyzed by HSA 50 μM (pH 7.40 ± 0.10, 37 ± 0.2°C)

No.	Nicotinate	k_HSA ± SD (10⁴ · min⁻¹)	Velocities ± SD (10⁴ · mmol/min per mg HSA)
1	Methyl	1.2 ± 0.17	0.188 ± 0.0082
2	Ethyl	1.5 ± 0.25	0.18 ± 0.018
3	n-Propyl	14.8 ± 0.61	1.32 ± 0.046
4	n-Butyl	56 ± 2.0	5.3 ± 0.27
5	Isobutyl	29 ± 2.2	2.9 ± 0.17
6	tert-Butyl	—^1-a	0.14 ± 0.033
7	n-Hexyl	32 ± 1.5	2.9 ± 0.15
8	2-Methoxyethyl	22.8 ± 0.89	2.19 ± 0.049
9	2-Butoxyethyl	504 ± 7.1	45 ± 1.0
10	Carbamoylmethyl	1.8 ± 0.76	0.54 ± 0.043
11	1-Carbamoylethyl	—^1-b	0.154 ± 0.0021
12	2-(N,N-diethylamino)ethyl	410 ± 6.2	41.3 ± 0.45
13	2-Tetrahydrofurfuryl	231 ± 8.8	21.2 ± 0.38
14	Benzyl	66 ± 2.3	6.7 ± 0.38
15	2-Phenoxyethyl	39 ± 2.3	3.68 ± 0.021
16	Phenyl	224 ± 2.0	21.3 ± 0.50
17	p-Fluorophenyl	51 ± 5.4	4.7 ± 0.44
18	2-Methoxyphenyl	8 ± 1.0	1.29 ± 0.035