Comparison of catalytic activity and P450 content between the baculovirus system and the human lymphoblastoid expression system
| System | Fraction | Expressed Protein | Coumarin 7-Hydroxylase Activity | P450 Content | Reductase Activity | |
|---|---|---|---|---|---|---|
| pmol/min/mg | pmol/min/pmol | |||||
| pmol/mg | nmol/min/mg | |||||
| Insect | Cell lysate | CYP2A6 | 120 | 0.31 | 388 | 55 |
| OR | — | — | — | 2849 | ||
| 2A6/OR | 1312 | 5.27 | 249 | 520 | ||
| Lymphoblastoid | Microsomes | CYP2A6 | 770 | 22.0 | 35 | 29 |
| OR | — | — | — | 3052 | ||
Insect cells were infected separately with v2A6 at a MOI of 0.02 or vOR at a MOI of 0.03. Insect cells coinfected at a MOI of v2A6/vOR of 0.015/0.001. Cells were harvested 72 hr postinfection, and lysates were assayed as described in Materials and Methods. Lymphoblastoid microsomes were isolated and assayed for activity as described previously (20).