Compound (GC Retention Time) | Ions (m/z) Observed on GC/MS (Fragment; Relative Intensity) |
---|---|
TDA (4.9 min) | 45 (100%); 59 (21.9%); 91 (18.8%); 119 (M+· − COOCH3; 32.8%); 146 (M+· − HOCH3; 45.3%); 178 [molecular ion (M+·); 20.3%] |
2-HEMA (7.4 min) | 45 (54.5%); 59 (22.9%); 85 (27.7%); 88 (58.1%); 103 (20.5%); 117 (M+· − [HOCH2CH2 and COOCH3] or M+· − [HOCH2CH2 and CH3CONH2]; 19%); 144 (M+· − HOCH2CH2S; 100%); 162 (M+· − COOCH3 and/or M+· − CH3CONH2; 7.5%); 203 (M+· − H2O; 13%) |
Both TDA and 2-HEMA were detected as their methyl esters after derivatization of extracted urine samples as described inMaterials and Methods.