Trypticase Soy Broth | Yeast Extract Broth | |||||
---|---|---|---|---|---|---|
Conc. of Drug and Metabolite | Time Required to Reach this Conc. | % of Drug Added | Conc. of Drug and Metabolite | Time Required to Reach this Conc. | % of Drug Added | |
μg/ml | (hr) | μg/ml | (hr) | |||
S-(+)-Mexiletine addition | ||||||
S-(+)-mexiletine remaining | 0.0 | 20 | 0 | 30.0 | 75 | 19 |
S-HMM produced | 0.1 | 75 | 0.06 | 2.2 | 75 | 1.3 |
S-PHM produced | 33.4 | 75 | 21 | 30.9 | 75 | 20 |
R-(−)-Mexiletine addition | ||||||
R-(−)-mexiletine remaining | 0 | 30 | 0 | 23.4 | 75 | 21 |
R-HMM produced | 1.0 | 69 | 0.7 | 2.0 | 75 | 1.2 |
R-PHM produced | 82.4 | 60 | 51 | 44.1 | 69 | 27 |
Ratio ofS-(+)-mex/R-(−)-mex | 0 | 1.29 | ||||
Ratio of S-HMM/R-HMM | 0.01 | 1.08 | ||||
Ratio ofS-PHM/R-PHM | 0.41 | 0.70 |
Summary of data obtained from the metabolism of authenticS-(+)-mexiletine and R-(−)-mexiletine standards in yeast extract broth and trypticase soy broth.
Conc., concentration; mex, mexiletine.