Effects of α-naphthoflavone on 7-ethoxyresorufin O-deethylation activities catalyzed by recombinant human CYP1A1, CYP1A2, and CYP1B1
| P450 | α-Naphthoflavone (μM) | 7-Ethoxyresorufin O-deethylation | |
|---|---|---|---|
| (pmol/min/nmol P450) | (%) | ||
| CYP1A1 | 0 | 13600 ± 2590 | (100) |
| 10 | 295 ± 20 | 2.2 | |
| 20 | 189 ± 31 | 1.4 | |
| 40 | 109 ± 29 | 0.8 | |
| CYP1A2 | 0 | 311 ± 20 | (100) |
| 10 | 11 ± 4 | 3.5 | |
| 20 | 9 ± 5 | 3.1 | |
| 40 | 10 ± 4 | 3.2 | |
| CYP1B1 | 0 | 1321 ± 121 | (100) |
| 10 | 41 ± 21 | 3.1 | |
| 20 | 32 ± 6 | 2.4 | |
| 40 | 17 ± 6 | 1.3 | |
Values represent means and SD of triplicate determinations.
(%) indicates percent of control values when the activities in the absence of α-naphthoflavone were determined to be 100%.