Apparent Michaelis-Menten parameters (± SE of determination) for theN-despropylation and hydroxylation of ropinirole in microsomes from three human livers
| N-despropylation | H32 | H99 | H102 |
|---|---|---|---|
| Vmax1 (nmol/hr/mg protein) | 420 ± 30 | 930 ± 140 | 220 ± 7.4 |
| K M1(μM) | 2000 ± 430 | 4100 ± 930 | 2000 ± 240 |
| V max2 (nmol/hr/mg protein) | 16 ± 13 | 2.2 ± 3.2 | 5.3 ± 4.2 |
| K M2 (μM) | 48 ± 50 | 4.9 ± 12 | 51 ± 54 |
| Hydroxylation | H32 | H99 | H102 |
|---|---|---|---|
| V max1 (nmol/hr/mg protein) | 330 ± 50 | 390 ± 20 | 46 ± 10 |
| K M1 (μM) | 6200 ± 1800 | 3900 ± 570 | 1700 ± 800 |
| V max2 (nmol/hr/mg protein) | 3.7 ± 3.9 | 17 ± 6.3 | 0.1 ± 0.9 |
| K M2 (μM) | 43 ± 66 | 87 ± 39 | 7.6 ± 87 |