Kinetic parameter values* for the hydroxylation/demethylation of nortriptyline by CYP isozymes
| Reaction | Kinetic Parameters | CYP Isozymes | ||
|---|---|---|---|---|
| 1A2 | 2C19 | 2D6 | ||
| (E)-10-hydroxylation | Km (μmol/l) | 0.74 (0.12) | ||
| V m(mol/hr/mol CYP) | 130 (16) | |||
| Cl int (106 1 hr/mol CYP) | 179 (6.6) | |||
| Demethylation | Km (μmol/l) | 54.2 (6.7) | 118 (2.7) | 0.48 (0.08) |
| V m(mol/hr/mol CYP) | 6.8 (0.9) | 93.1 (2.6) | 19.4 (2.1) | |
| Cl int (106 1 hr/mol CYP) | 0.12 (0.003) | 0.79 (0.04) | 41.7 (5.9) | |
* Mean values with SE in parentheses; N = 3 independent series.