Table 2

Purification of human FMO3 from transfected T. ni cells

	Total Activity^2-a	Yield	Protein	Specific Activity^2-a
	nmol/min	%	mg	nmol/mg/min
Membranes	7,560	100	154	49
Octyl-Sepharose pool	1,570	21	—	—
DEAE-Sepharose pool	860	11	—	—
Hydroxyapatite pool	600	8^2-b	3	201

↵2-a Reaction pathway monitored was the sulfoxidation of methyl p-tolyl sulfide with a substrate concentration of 1 mM.
↵2-b Based on FAD content, a total of 18 nmol of holo-FMO3 was recovered.