Hepatocyte scaling factors (hepatocellularity) determined using protein and DNA methodology in UT, PB-treated, and DEX-treated rats
| Treatment | DNA Content of Liver | DNA Content of Cells | Hepatocellularity2-a | Protein Content of Liver | Protein Content of Cells | Hepatocellularity2-b* |
|---|---|---|---|---|---|---|
| mg/g | μg/106cells | 106 hepatocytes/g liver | mg/g | mg/106 cells | 106hepatocytes/g liver | |
| UT | 2.7 ± 0.3 | 23.7 ± 2.8 | 85 | 163.5 ± 8.0 | 1.5 ± 0.2 | 109 |
| PB | 2.6 ± 0.1 | 26.8 ± 5.0 | 73 | 163.4 ± 4.8 | 1.5 ± 0.2 | 109 |
| DEX | 2.7 ± 0.1 | 21.5 ± 4.0 | 94 | 175.6 ± 9.6 | 1.4 ± 0.1 | 125 |
Data are presented as means ± SD (N = 4 for each group). There is no statistically significant difference between either set of UT, PB, or DEX values. Protein hepatocellularity is significantly different from DNA hepatocellularity (* p < 0.05).
↵2-a Hepatocellularity calculated using DNA methodology based on ratio of liver/cell DNA content, with a correction factor of 0.75 as discussed in Materials and Methods.
↵2-b Hepatocellularity calculated using protein methodology based on ratio of liver/cell protein.