Metabolism of testosterone in liver microsomes pre-incubated with L-694,458 and NADPH
| L-694,458 (μM) | Testosterone (nmoles remaining in 30 min) | 6β-OH-Testosterone | Androstenedione |
|---|---|---|---|
| (nmoles produced in 30 min) | |||
| I. Rhesus monkey | |||
| Control | 27 | 47 | 9 |
| 10 | 51 | 8 | 19 |
| 100 | 65 | 3 | 25 |
| II. Human | |||
| Control | 50 | 25 | 5 |
| 10 | 88 | 3 | 3 |
| 100 | 90 | 3 | 4 |
Microsomes (2 mg/ml) were pre-incubated with L-694,458 or solvent (control) in the presence of an NADPH-regenerating system at 37°C for 30 min. Testosterone (100 μM) was added and incubations continued for an additional 30 min. The amounts of testosterone remaining and metabolites formed were determined by HPLC analysis.